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Bluegene Biotech
Products >> BlueGene Biotech Hamster Total Adiponectin ELISA k
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BlueGene Biotech Hamster Total Adiponectin ELISA k
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hamster elisa, |
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E18A0125 Hamster Total Adiponectin ELISA kit
Hamster ELISA kit is suitable for the ***ection of samples from Hamster species. Total Adiponectin can also be called 30 kDa adipocyte complement related protein, 30 kDa adipocyte complement-related protein, ACDC, ACRP 30, ACRP30, ADIPO, Adipocyte, Adipocyte C1q and collagen domain containing protein, Adipocyte complement related 30 kDa protein, Adipocyte complement related protein of 30 kDa, Adipocyte complement-related 30 kDa protein, Adiponectin, AdipoQ, Adipose most abundant gene transcript 1, Adipose most abundant gene transcript 1 protein, Adipose specific collagen like factor, ADIPQTL1, ADPN, APM 1, apM-1, ApM1, C1q and collagen domain-containing protein, GBP 28, GBP28, Gelatin binding protein, Gelatin binding protein 28, Gelatin-binding protein.
Principle of the Assay
ADPN ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ADPN antibody and an ADPN-HRP conjugate. The assay sample and buffer are incubated together with ADPN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADPN concentration since ADPN from samples and ADPN-HRP conjugate compete for the anti-ADPN antibody binding site. Since the number of sites is limited, as more sites are occupied by ADPN from the sample, fewer sites are left to bind ADPN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADPN concentration in each sample is interpolated from this standard curve.
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| Company: |
Bluegene Biotech
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| Contact: |
Blue gene |
| Address: |
Lane 166 Tianxiong Road(Rm.403,404, Bldg 2),Pudong NewDistrict,Shanghai |
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| Tel: |
400-882-6373 |
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400-882-6373 |
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