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Product Name: |
Human/Canine/Porcine Insulin ELISA kit
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Related proudcts |
human/canine/porcine insulin e, |
Specifications |
Human/Canine/Porcine Insulin ELISA kit |
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NE01I0004 Human/Canine/Porcine elisa insulin kit
Human/Canine/Porcine elisa for insulin kit is suitable for the ***ection of samples from human species. Insulin can also be called ILPR, Insulin A chain, Insulin B chain, IRDN, INS, IDDM, IDDM1, IDDM2, ILPR, MODY10, insulin, PNDM4.
Principle of the Assay
This elisa kits for insulin measurement is applied to the solid phase sandwich Enzyme Linked Immunosorbent Assay. The microtiter plate has been pre-coated with capture antibody. The antigen to be measured and the biotin-conjugated ***ection antibody are then added to the microtiter plate wells sequentially to form a capture antibody-antigen-***ection antibody complex. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP, and finally turns to yellow immediately after adding the stop solution. Color of TMB substrate positively correlated with total insulin bound in the initial steps. The color is measured by spectrophotometrically with wavelength of 450nm. The concentration of insulin in samples is then ***ermined by co***ring the O.D. of the samples to the standard curve.
We provide bluegene elisa kit, igfbp 1 elisa, igfbp1 elisa kit, human igfbp7 elisa, elisa insulin assay, human insulin elisa, cho hcp elisa kit, etc. For more information, please feel free to contact us!
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Company: |
Bluegene Biotech
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Contact: |
Blue gene |
Address: |
Lane 166 Tianxiong Road(Rm.403,404, Bldg 2),Pudong NewDistrict,Shanghai |
Postcode: |
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Tel: |
400-882-6373 |
Fax: |
400-882-6373 |
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